LOCUS       40924_5244       11948 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Gateway vector pB4cRGW DNA, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11948)
  AUTHORS   Kamigaki A, Nito K, Hikino K, Goto-Yamada S, Nishimura M, Nakagawa 
            T, Mano S.
  TITLE     Gateway Vectors for Simultaneous Detection of Multiple 
            Protein-Protein Interactions in Plant Cells Using Bimolecular 
            Fluorescence Complementation
  JOURNAL   PLoS ONE 11 (8), E0160717 (2016)
  PUBMED    27490375
REFERENCE   2  (bases 1 to 11948)
  AUTHORS   Mano S, Nakagawa T.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JUL-2013) Contact:Shoji Mano National Institute for 
            Basic Biology, Department of Cell Biology; 38 Nishigonaka, Myodaiji,
            Okazaki, Aichi 444-8585, Japan
REFERENCE   3  (bases 1 to 11948)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 11948)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS ONE"; 
            date: "2016"; volume: "11"; issue: "8"; pages: "E0160717"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (10-JUL-2013) Contact:Shoji Mano National Institute for Basic 
            Biology, Department of Cell Biology; 38 Nishigonaka, Myodaiji, 
            Okazaki, Aichi 444-8585, Japan"
COMMENT     SGRef: number: 3; type: "Journal Article"
COMMENT     These colnes were obtained at our laboratory.
FEATURES             Location/Qualifiers
     source          1..11948
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    complement(114..138)
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     primer_bind     341..357
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        872..1217
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     CDS             1241..1267
                     /codon_start=1
                     /label=HA
                     /note="HA (human influenza hemagglutinin) epitope tag"
                     /translation="YPYDVPDYA"
     protein_bind    1489..1613
                     /label=attR1
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        1666..1696
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an 'up' mutation"
     CDS             1750..2406
                     /codon_start=1
                     /label=CmR
                     /note="chloramphenicol acetyltransferase"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHSCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     CDS             2751..3053
                     /codon_start=1
                     /label=ccdB
                     /note="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     protein_bind    complement(3097..3221)
                     /label=attR2
                     /note="recombination site for the Gateway(R) LR reaction"
     terminator      3247..3499
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     primer_bind     complement(3533..3549)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    3557..3573
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(3581..3611)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(3626..3647)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     terminator      complement(3681..3933)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(4326..5114)
                     /codon_start=1
                     /label=NeoR/KanR
                     /note="aminoglycoside phosphotransferase from Tn5"
                     /translation="IEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRPV
                     LFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLSS
                     HLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQG
                     LAPAELFARLKARMPDGDDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIAL
                     ATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     promoter        complement(5138..5321)
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     misc_feature    complement(5527..5551)
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             6079..6867
                     /codon_start=1
                     /label=SmR
                     /note="aminoglycoside adenylyltransferase (Murphy, 1985)"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      7114..7702
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     misc_feature    complement(7888..8028)
                     /label=bom
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(8372..8566)
                     /direction=LEFT
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(8635..9699)
                     /codon_start=1
                     /label=pVS1 RepA
                     /note="replication protein from the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
                     /translation="GRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESWQA
                     AADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLSKR
                     DRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKPGR
                     VFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGEAL
                     ISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYRLA
                     RRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPILV
                     MRYRNLIEGEASAGS"
     CDS             complement(10136..10762)
                     /codon_start=1
                     /label=pVS1 StaA
                     /note="stability protein from the Pseudomonas plasmid pVS1
                     (Heeb et al., 2000)"
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
ORIGIN
        1 ttgatcccga ggggaaccct gtggttggca tgcacataca aatggacgaa cggataaacc
       61 ttttcacgcc cttttaaata tccgttattc taataaacgc tcttttctct taggtttacc
      121 cgccaatata tcctgtcaaa cactgatagt ttaaactgaa ggcgggaaac gacaatctga
      181 tccaagctca agctgctcta gcattcgcca ttcaggctgc gcaactgttg ggaagggcga
      241 tcggtgcggg cctcttcgct attacgccag ctggcgaaag ggggatgtgc tgcaaggcga
      301 ttaagttggg taacgccagg gttttcccag tcacgacgtt gtaaaacgac ggccagtgcc
      361 aagcttgcat gcctgcaggt ccccagatta gccttttcaa tttcagaaag aatgctaacc
      421 cacagatggt tagagaggct tacgcagcag gtctcatcaa gacgatctac ccgagcaata
      481 atctccagga aatcaaatac cttcccaaga aggttaaaga tgcagtcaaa agattcagga
      541 ctaactgcat caagaacaca gagaaagata tatttctcaa gatcagaagt actattccag
      601 tatggacgat tcaaggcttg cttcacaaac caaggcaagt aatagagatt ggagtctcta
      661 aaaaggtagt tcccactgaa tcaaaggcca tggagtcaaa gattcaaata gaggacctaa
      721 cagaactcgc cgtaaagact ggcgaacagt tcatacagag tctcttacga ctcaatgaca
      781 agaagaaaat cttcgtcaac atggtggagc acgacacact tgtctactcc aaaaatatca
      841 aagatacagt ctcagaagac caaagggcaa ttgagacttt tcaacaaagg gtaatatccg
      901 gaaacctcct cggattccat tgcccagcta tctgtcactt tattgtgaag atagtggaaa
      961 aggaaggtgg ctcctacaaa tgccatcatt gcgataaagg aaaggccatc gttgaagatg
     1021 cctctgccga cagtggtccc aaagatggac ccccacccac gaggagcatc gtggaaaaag
     1081 aagacgttcc aaccacgtct tcaaagcaag tggattgatg tgatatctcc actgacgtaa
     1141 gggatgacgc acaatcccac tatccttcgc aagacccttc ctctatataa ggaagttcat
     1201 ttcatttgga gagaacacgg gggactctag aatgagcatg tacccatacg atgttcctga
     1261 ctatgcgggc gccctgaagg gcgagatcaa gatgaggctg aagctgaagg acggcggcca
     1321 ctacgacgcc gaggtcaaga ccacctacat ggccaagaag cccgtgcagc tgcccggcgc
     1381 ctacaagacc gacatcaagc tggacatcac ctcccacaac gaggactaca ccatcgtgga
     1441 acagtacgag cgcgccgagg gccgccactc caccggcgcc ctgttatcac aagtttgtac
     1501 aaaaaagctg aacgagaaac gtaaaatgat ataaatatca atatattaaa ttagattttg
     1561 cataaaaaac agactacata atactgtaaa acacaacata tccagtcact atggcggccc
     1621 ctcgaggcct cgagccctcg aggggccgca ttaggcaccc caggctttac actttatgct
     1681 tccggctcgt ataatgtgtg gattttgagt taggatccgt cgagattttc aggagctaag
     1741 gaagctaaaa tggagaaaaa aatcactgga tataccaccg ttgatatatc ccaatggcat
     1801 cgtaaagaac attttgaggc atttcagtca gttgctcaat gtacctataa ccagaccgtt
     1861 cagctggata ttacggcctt tttaaagacc gtaaagaaaa ataagcacaa gttttatccg
     1921 gcctttattc acattcttgc ccgcctgatg aatgctcatc cggaattccg tatggcaatg
     1981 aaagacggtg agctggtgat atgggatagt gttcactctt gttacaccgt tttccatgag
     2041 caaactgaaa cgttttcatc gctctggagt gaataccacg acgatttccg gcagtttcta
     2101 cacatatatt cgcaagatgt ggcgtgttac ggtgaaaacc tggcctattt ccctaaaggg
     2161 tttattgaga atatgttttt cgtctcagcc aatccctggg tgagtttcac cagttttgat
     2221 ttaaacgtgg ccaatatgga caacttcttc gcccccgttt tcaccatggg caaatattat
     2281 acgcaaggcg acaaggtgct gatgccgctg gcgattcagg ttcatcatgc cgtctgtgat
     2341 ggcttccatg tcggcagaat gcttaatgaa ttacaacagt actgcgatga gtggcagggc
     2401 ggggcgtaaa cgcgtggatc cggcttacta aaagccagat aacagtatgc gtatttgcgc
     2461 gctgattttt gcggtataag aatatatact gatatgtata cccgaagtat gtcaaaaaga
     2521 ggtgtgctat gaagcagcgt attacagtga cagttgacag cgacagctat cagttgctca
     2581 aggcatatat gatgtcaata tctccggtct ggtaagcaca accatgcaga atgaagcccg
     2641 tcgtctgcgt gccgaacgct ggaaagcgga aaatcaggaa gggatggctg aggtcgcccg
     2701 gtttattgaa atgaacggct cttttgctga cgagaacagg gactggtgaa atgcagttta
     2761 aggtttacac ctataaaaga gagagccgtt atcgtctgtt tgtggatgta cagagtgata
     2821 ttattgacac gcccgggcga cggatggtga tccccctggc cagtgcacgt ctgctgtcag
     2881 ataaagtctc ccgtgaactt tacccggtgg tgcatatcgg ggatgaaagc tggcgcatga
     2941 tgaccaccga tatggccagt gtgccggtct ccgttatcgg ggaagaagtg gctgatctca
     3001 gccaccgcga aaatgacatc aaaaacgcca ttaacctgat gttctgggga atataaatgt
     3061 caggctccct tatacacagc cagtctgcag gtcgaccata gtgactggat atgttgtgtt
     3121 ttacagtatt atgtagtctg ttttttatgc aaaatctaat ttaatatatt gatatttata
     3181 tcattttacg tttctcgttc agctttcttg tacaaagtgg tgataaccta gagctcgaat
     3241 ttccccgatc gttcaaacat ttggcaataa agtttcttaa gattgaatcc tgttgccggt
     3301 cttgcgatga ttatcatata atttctgttg aattacgtta agcatgtaat aattaacatg
     3361 taatgcatga cgttatttat gagatgggtt tttatgatta gagtcccgca attatacatt
     3421 taatacgcga tagaaaacaa aatatagcgc gcaaactagg ataaattatc gcgcgcggtg
     3481 tcatctatgt tactagatcg ggaattggtt ccggaaccaa ttcgtaatca tggtcatagc
     3541 tgtttcctgt gtgaaattgt tatccgctca caattccaca caacatacga gccggaagca
     3601 taaagtgtaa agcctggggt gcctaatgag tgagctaact cacattaggc tgaattaggc
     3661 gcgcctattt ctgaattccc gatctagtaa catagatgac accgcgcgcg ataatttatc
     3721 ctagtttgcg cgctatattt tgttttctat cgcgtattaa atgtataatt gcgggactct
     3781 aatcataaaa acccatctca taaataacgt catgcattac atgttaatta ttacatgctt
     3841 aacgtaattc aacagaaatt atatgataat catcgcaaga ccggcaacag gattcaatct
     3901 taagaaactt tattgccaaa tgtttgaacg atcggggatc atccgggtct gtggcgggaa
     3961 ctccacgaaa atatccgaac gcagcaagat atcgcggtgc atctcggtct tgcctgggca
     4021 gtcgccgccg acgccgttga tgtggacgcc gggcccgatc atattgtcgc tcaggatcgt
     4081 ggcgttgtgc ttgtcggccg ttgctgtcgt aatgatatcg gcaccttcga ccgcctgttc
     4141 cgcagagatc ccgtgggcga agaactccag catgagatcc ccgcgctgga ggatcatcca
     4201 gccggcgtcc cggaaaacga ttccgaagcc caacctttca tagaaggcgg cggtggaatc
     4261 gaaatctcgt gatggcaggt tgggcgtcgc ttggtcggtc atttcgaacc ccagagtccc
     4321 gctcagaaga actcgtcaag aaggcgatag aaggcgatgc gctgcgaatc gggagcggcg
     4381 ataccgtaaa gcacgaggaa gcggtcagcc cattcgccgc caagctcttc agcaatatca
     4441 cgggtagcca acgctatgtc ctgatagcgg tccgccacac ccagccggcc acagtcgatg
     4501 aatccagaaa agcggccatt ttccaccatg atattcggca agcaggcatc gccatgggtc
     4561 acgacgagat catcgccgtc gggcatgcgc gccttgagcc tggcgaacag ttcggctggc
     4621 gcgagcccct gatgctcttc gtccagatca tcctgatcga caagaccggc ttccatccga
     4681 gtacgtgctc gctcgatgcg atgtttcgct tggtggtcga atgggcaggt agccggatca
     4741 agcgtatgca gccgccgcat tgcatcagcc atgatggata ctttctcggc aggagcaagg
     4801 tgagatgaca ggagatcctg ccccggcact tcgcccaata gcagccagtc ccttcccgct
     4861 tcagtgacaa cgtcgagcac agctgcgcaa ggaacgcccg tcgtggccag ccacgatagc
     4921 cgcgctgcct cgtcctgcag ttcattcagg gcaccggaca ggtcggtctt gacaaaaaga
     4981 accgggcgcc cctgcgctga cagccggaac acggcggcat cagagcagcc gattgtctgt
     5041 tgtgcccagt catagccgaa tagcctctcc acccaagcgg ccggagaacc tgcgtgcaat
     5101 ccatcttgtt caatcatgcg aaacgatcca gatccggtgc agattatttg gattgagagt
     5161 gaatatgaga ctctaattgg ataccgaggg gaatttatgg aacgtcagtg gagcattttt
     5221 gacaagaaat atttgctagc tgatagtgac cttaggcgac ttttgaacgc gcaataatgg
     5281 tttctgacgt atgtgcttag ctcattaaac tccagaaacc cgcggctgag tggctccttc
     5341 aacgttgcgg ttctgtcagt tccaaacgta aaacggcttg tcccgcgtca tcggcggggg
     5401 tcataacgtg actcccttaa ttctccgctc atgatgaatt cagaaataaa ttcagcctaa
     5461 ttcggcgtta attcagtaca ttaaaaacgt ccgcaatgtg ttattaagtt gtctaagcgt
     5521 caatttgttt acaccacaat atatcctgcc accagccagc caacagctcc ccgaccggca
     5581 gctcggcaca aaatcaccac tcgatacagg cagcccatca gtccgggacg gcgtcagcgg
     5641 gagagccgtt gtaaggcggc agactttgct catgttaccg atgctattcg gaagaacggc
     5701 aactaagctg ccgggtttga aacacggatg atctcgcgga gggtagcatg ttgattgtaa
     5761 cgatgacaga gcgttgctgc ctgtgatcaa ttcgggcacg aacccagtgg acataagcct
     5821 cgttcggttc gtaagctgta atgcaagtag cgtaactgcc gtcacgcaac tggtccagaa
     5881 ccttgaccga acgcagcggt ggtaacggcg cagtggcggt tttcatggct tcttgttatg
     5941 acatgttttt ttggggtaca gtctatgcct cgggcatcca agcagcaagc gcgttacgcc
     6001 gtgggtcgat gtttgatgtt atggagcagc aacgatgtta cgcagcaggg cagtcgccct
     6061 aaaacaaagt taaacatcat gggggaagcg gtgatcgccg aagtatcgac tcaactatca
     6121 gaggtagttg gcgtcatcga gcgccatctc gaaccgacgt tgctggccgt acatttgtac
     6181 ggctccgcag tggatggcgg cctgaagcca cacagtgata ttgatttgct ggttacggtg
     6241 accgtaaggc ttgatgaaac aacgcggcga gctttgatca acgacctttt ggaaacttcg
     6301 gcttcccctg gagagagcga gattctccgc gctgtagaag tcaccattgt tgtgcacgac
     6361 gacatcattc cgtggcgtta tccagctaag cgcgaactgc aatttggaga atggcagcgc
     6421 aatgacattc ttgcaggtat cttcgagcca gccacgatcg acattgatct ggctatcttg
     6481 ctgacaaaag caagagaaca tagcgttgcc ttggtaggtc cagcggcgga ggaactcttt
     6541 gatccggttc ctgaacagga tctatttgag gcgctaaatg aaaccttaac gctatggaac
     6601 tcgccgcccg actgggctgg cgatgagcga aatgtagtgc ttacgttgtc ccgcatttgg
     6661 tacagcgcag taaccggcaa aatcgcgccg aaggatgtcg ctgccgactg ggcaatggag
     6721 cgcctgccgg cccagtatca gcccgtcata cttgaagcta gacaggctta tcttggacaa
     6781 gaagaagatc gcttggcctc gcgcgcagat cagttggaag aatttgtcca ctacgtgaaa
     6841 ggcgagatca ccaaggtagt cggcaaataa tgtctagcta gaaattcgtt caagccgacg
     6901 ccgcttcgcc ggcgttaact caagcgatta gatgcactaa gcacataatt gctcacagcc
     6961 aaactatcag gtcaagtctg cttttattat ttttaagcgt gcataataag ccctacacaa
     7021 attgggagat atatcatgca tgaccaaaat cccttaacgt gagttttcgt tccactgagc
     7081 gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat
     7141 ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga
     7201 gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt
     7261 ccttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata
     7321 cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac
     7381 cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg
     7441 ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg
     7501 tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag
     7561 cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct
     7621 ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc
     7681 aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt
     7741 ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg
     7801 tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga
     7861 gtcagtgagc gaggaagcgg aagagcgcct gatgcggtat tttctcctta cgcatctgtg
     7921 cggtatttca caccgcatat ggtgcactct cagtacaatc tgctctgatg ccgcatagtt
     7981 aagccagtat acactccgct atcgctacgt gactgggtca tggctgcgcc ccgacacccg
     8041 ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc ttacagacaa
     8101 gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc
     8161 gcgaggcagg gtgccttgat gtgggcgccg gcggtcgagt ggcgacggcg cggcttgtcc
     8221 gcgccctggt agattgcctg gccgtaggcc agccattttt gagcggccag cggccgcgat
     8281 aggccgacgc gaagcggcgg ggcgtaggga gcgcagcgac cgaagggtag gcgctttttg
     8341 cagctcttcg gctgtgcgct ggccagacag ttatgcacag gccaggcggg ttttaagagt
     8401 tttaataagt tttaaagagt tttaggcgga aaaatcgcct tttttctctt ttatatcagt
     8461 cacttacatg tgtgaccggt tcccaatgta cggctttggg ttcccaatgt acgggttccg
     8521 gttcccaatg tacggctttg ggttcccaat gtacgtgcta tccacaggaa agagaccttt
     8581 tcgacctttt tcccctgcta gggcaatttg ccctagcatc tgctccgtac attaggaacc
     8641 ggcggatgct tcgccctcga tcaggttgcg gtagcgcatg actaggatcg ggccagcctg
     8701 ccccgcctcc tccttcaaat cgtactccgg caggtcattt gacccgatca gcttgcgcac
     8761 ggtgaaacag aacttcttga actctccggc gctgccactg cgttcgtaga tcgtcttgaa
     8821 caaccatctg gcttctgcct tgcctgcggc gcggcgtgcc aggcggtaga gaaaacggcc
     8881 gatgccggga tcgatcaaaa agtaatcggg gtgaaccgtc agcacgtccg ggttcttgcc
     8941 ttctgtgatc tcgcggtaca tccaatcagc tagctcgatc tcgatgtact ccggccgccc
     9001 ggtttcgctc tttacgatct tgtagcggct aatcaaggct tcaccctcgg ataccgtcac
     9061 caggcggccg ttcttggcct tcttcgtacg ctgcatggca acgtgcgtgg tgtttaaccg
     9121 aatgcaggtt tctaccaggt cgtctttctg ctttccgcca tcggctcgcc ggcagaactt
     9181 gagtacgtcc gcaacgtgtg gacggaacac gcggccgggc ttgtctccct tcccttcccg
     9241 gtatcggttc atggattcgg ttagatggga aaccgccatc agtaccaggt cgtaatccca
     9301 cacactggcc atgccggccg gccctgcgga aacctctacg tgcccgtctg gaagctcgta
     9361 gcggatcacc tcgccagctc gtcggtcacg cttcgacaga cggaaaacgg ccacgtccat
     9421 gatgctgcga ctatcgcggg tgcccacgtc atagagcatc ggaacgaaaa aatctggttg
     9481 ctcgtcgccc ttgggcggct tcctaatcga cggcgcaccg gctgccggcg gttgccggga
     9541 ttctttgcgg attcgatcag cggccgcttg ccacgattca ccggggcgtg cttctgcctc
     9601 gatgcgttgc cgctgggcgg cctgcgcggc cttcaacttc tccaccaggt catcacccag
     9661 cgccgcgccg atttgtaccg ggccggatgg tttgcgaccg tcacgccgat tcctcgggct
     9721 tgggggttcc agtgccattg cagggccggc agacaaccca gccgcttacg cctggccaac
     9781 cgcccgttcc tccacacatg gggcattcca cggcgtcggt gcctggttgt tcttgatttt
     9841 ccatgccgcc tcctttagcc gctaaaattc atctactcat ttattcattt gctcatttac
     9901 tctggtagct gcgcgatgta ttcagatagc agctcggtaa tggtcttgcc ttggcgtacc
     9961 gcgtacatct tcagcttggt gtgatcctcc gccggcaact gaaagttgac ccgcttcatg
    10021 gctggcgtgt ctgccaggct ggccaacgtt gcagccttgc tgctgcgtgc gctcggacgg
    10081 ccggcactta gcgtgtttgt gcttttgctc attttctctt tacctcatta actcaaatga
    10141 gttttgattt aatttcagcg gccagcgcct ggacctcgcg ggcagcgtcg ccctcgggtt
    10201 ctgattcaag aacggttgtg ccggcggcgg cagtgcctgg gtagctcacg cgctgcgtga
    10261 tacgggactc aagaatgggc agctcgtacc cggccagcgc ctcggcaacc tcaccgccga
    10321 tgcgcgtgcc tttgatcgcc cgcgacacga caaaggccgc ttgtagcctt ccatccgtga
    10381 cctcaatgcg ctgcttaacc agctccacca ggtcggcggt ggcccatatg tcgtaagggc
    10441 ttggctgcac cggaatcagc acgaagtcgg ctgccttgat cgcggacaca gccaagtccg
    10501 ccgcctgggg cgctccgtcg atcactacga agtcgcgccg gccgatggcc ttcacgtcgc
    10561 ggtcaatcgt cgggcggtcg atgccgacaa cggttagcgg ttgatcttcc cgcacggccg
    10621 cccaatcgcg ggcactgccc tggggatcgg aatcgactaa cagaacatcg gccccggcga
    10681 gttgcagggc gcgggctaga tgggttgcga tggtcgtctt gcctgacccg cctttctggt
    10741 taagtacagc gataaccttc atgcgttccc cttgcgtatt tgtttattta ctcatcgcat
    10801 catatacgca gcgaccgcat gacgcaagct gttttactca aatacacatc acctttttag
    10861 acggcggcgc tcggtttctt cagcggccaa gctggccggc caggccgcca gcttggcatc
    10921 agacaaaccg gccaggattt catgcagccg cacggttgag acgtgcgcgg gcggctcgaa
    10981 cacgtacccg gccgcgatca tctccgcctc gatctcttcg gtaatgaaaa acggttcgtc
    11041 ctggccgtcc tggtgcggtt tcatgcttgt tcctcttggc gttcattctc ggcggccgcc
    11101 agggcgtcgg cctcggtcaa tgcgtcctca cggaaggcac cgcgccgcct ggcctcggtg
    11161 ggcgtcactt cctcgctgcg ctcaagtgcg cggtacaggg tcgagcgatg cacgccaagc
    11221 agtgcagccg cctctttcac ggtgcggcct tcctggtcga tcagctcgcg ggcgtgcgcg
    11281 atctgtgccg gggtgagggt agggcggggg ccaaacttca cgcctcgggc cttggcggcc
    11341 tcgcgcccgc tccgggtgcg gtcgatgatt agggaacgct cgaactcggc aatgccggcg
    11401 aacacggtca acaccatgcg gccggccggc gtggtggtgt cggcccacgg ctctgccagg
    11461 ctacgcaggc ccgcgccggc ctcctggatg cgctcggcaa tgtccagtag gtcgcgggtg
    11521 ctgcgggcca ggcggtctag cctggtcact gtcacaacgt cgccagggcg taggtggtca
    11581 agcatcctgg ccagctccgg gcggtcgcgc ctggtgccgg tgatcttctc ggaaaacagc
    11641 ttggtgcagc cggccgcgtg cagttcggcc cgttggttgg tcaagtcctg gtcgtcggtg
    11701 ctgacgcggg catagcccag caggccagcg gcggcgctct tgttcatggc gtaatgtctc
    11761 cggttctagt cgcaagtatt ctactttatg cgactaaaac acgcgacaag aaaacgccag
    11821 gaaaagggca gggcggcagc ctgtcgcgta acttaggact tgtgcgacat gtcgttttca
    11881 gaagacggct gcactgaacg tcagaagccg actgcactat agcagcggag gggttggatc
    11941 aaagtact
//
